Fig. 7. DJ-1 modulates ER stress-induced cleaved caspase-3 protein expression level.

a DJ-1 WT and KO mouse cortical neurons were collected, and  three independent experiments were conducted. Blots were probed with the indicated antibodies. b, c Three independent experiments were conducted as in A after treatment with DMSO (0 h) as vehicle control or Tun for up to 12 h (b) or 24 h (c). Blots were probed with the indicated antibodies and graphed by Prism 7 (***p < 0.001, ****p < 0.0001, two-way ANOVA with Tukey post-test, n = 3). d DJ-1 WT and KO brain tissues were collected from SN of 1-year-old mice. Blots were probed with the indicated antibodies and quantified by Image J and graphed by Prism 7. Right panel of d: Data were obtained from the same conditions and averaged to compare cleaved caspase-3 (*p < 0.05, paired two-tailed t-test, n = 4) protein levels between DJ-1 WT and KO in SN mice brain. β-Actin were used as a loading control