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. 2019 Feb 12;9:1827. doi: 10.1038/s41598-018-36140-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Hyperglycemia does not augment the glucose uptake of cardiac fibroblasts in vitro. Cultures of primary cardiac fibroblasts were treated with media containing 5.5 or 25 mM glucose supplemented with radiolabeled 2-deoxyglucose in 10% FBS for up to 72 hours ± 100 nM insulin. (a) Experimental design schematic. (b) Time course of radiolabeled glucose uptake (n = 4). (c) Quantification of HA secretion into the media (n = 4). Data represent mean ± SEM; two-way ANOVA with Sidak’s multiple-comparison correction (b) and one-way ANOVA with Sidak’s multiple-comparison correction (c).