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Immunopositivity (brown) of co-culture Caco-2 and HT29-MTX cells at percentages 90% Caco-2/10% HT29-MTX layered on L-pNIPAM hydrogel scaffolds under dynamic culture conditions following 7 weeks as control or for 6 weeks and then treated with 10 ng/ml IL-1β for 1 week under dynamic culture conditions or hypoxic at 1% O2 for 1 week under static culture conditions. Cell nuclei were stained with haematoxylin (blue). The black arrows indicate positively stained cells. Scale bar = 100 µm.
