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. 2019 Feb 12;9:1812. doi: 10.1038/s41598-019-38524-8

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Morphology of long term co-culture of Caco-2 and HT29-MTX cells at percentages: (A) 90% Caco-2/10% HT29-MTX; (B) 75% Caco-2/25% HT29-MTX layered on L-pNIPAM hydrogel scaffolds under dynamic culture conditions following 12 weeks as control or for 11 weeks and then treated with 10 ng/ml IL-1β or 10 ng/ml TNFα for 1 week under dynamic culture conditions or hypoxic at 1% O₂ for 1 week under static culture conditions stained with H&E and AB-PAS, blue: acidic mucin (red arrows); magenta: neutral mucin (black arrows). Scale bar = 100 µm