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. 2019 Feb 12;9:1835. doi: 10.1038/s41598-018-38455-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Myosin and actin in the neural plate. (a–d) Transverse confocal section of neural plate stained for phosho-myosin and F-actin. (e,e”) Horizontal confocal section through EVL stained for phospho-myosin and F-actin. (f,f”) Horizontal confocal section at level of dorsal surface of neural plate cells stained for phospho-myosin and F-actin. (g,g”) Horizontal confocal section 3 µm below level of dorsal surface of neural plate cells stained for phospho-myosin and F-actin. (h–j”) Myosin and actin revealed in living neural plate by Tg(actb1:myl12.1-GFP) and Tg(actb1:GFP-utrCH) transgenic embryos. Sections equivalent to panels (e–g”). Scale bars are 5 µm.