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. 2019 Feb 12;9:1837. doi: 10.1038/s41598-018-38320-w

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Comparison of EV morphology, size distribution, and yield with different isolation techniques. (a) Representative photos of AFSC-EV morphology analyzed by TEM; the two different magnifications highlight morphology of individual EVs at near fields (left column) and a population of EVs at far fields (right column) Scale bar = 200 nm. (UC = Ultracentrifugation; TEIR = Total Exosome Isolation Reagent). B: Representative plot of the average size distributions of AFSC-EVs isolated with the different techniques visualized using Nanoparticle Tracking Analysis. Data are representative of eight videos of AFSC-EV preparations. X-axis = size distribution (nm), y-axis = concentration (particles/ml). (c) Total particle yield calculated as the area under the curve from (b) of each AFSC-EV preparation.