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. 2019 Feb 12;51(2):12. doi: 10.1038/s12276-019-0205-7

Fig. 9. The effects of (2R)-pterosin B and (2R,3R)-pteroside C on the cellular secretion of Aβ peptides.

Fig. 9

a Effect of (2R)-pterosin B on the secretion of Aβ peptides. APPswe-secreting neuroblastoma cells were treated with 12, 60, 250, or 500 µM of (2R)-pterosin B for 24 h, and conditioned media were collected in the presence of protease inhibitor. β-SI (10 µM) was used as positive control. Negative control included cells cultured in the absence of test compounds. Quantitative analysis of secreted Aβ40 and Aβ42 in the conditioned media was performed using sandwich ELISA. The means ± SD from three independent experiments are shown. The secreted Aβ40 peptides significantly decreased in the presence of 500 µM (2R)-pterosin B. CON negative control, β-SI β-secretase inhibitor III, Aβ β-amyloid. *P < 0.05, **P < 0.01. b Effect of (2R,3R)-pteroside C on the secretion of Aβ peptides. APPswe-secreting neuroblastoma cells were treated with 12, 60, 125, 250, or 500 µM of (2R,3R)-pteroside C for 24 h, and conditioned media were collected in the presence of protease inhibitor. β-SI (10 µM) was used as positive control. Negative control included cells cultured in the absence of test compounds. Quantitative analysis of secreted Aβ40 and Aβ42 in the conditioned media was performed using sandwich ELISA. The means ± SD from three independent experiments are shown. The secreted Aβ42 peptide significantly decreased in the presence of 500 µM (2R,3R)-pteroside C. CON negative control, β-SI β-secretase inhibitor III, Aβ β-amyloid. *P < 0.05