Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 12;9:1839. doi: 10.1038/s41598-018-37949-x

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

sgRNA targeting the coding strand of dnaK gene prevents leptospiral growth in plates. L. biflexa cells were transformed with the plasmid pMaOri.dCas9 alone or containing sgRNA targeting the coding (sgRNAdnaK1) or template (sgRNAdnaK2) strand of the dnaK gene. Colonies were counted (A) and DnaK protein levels were evaluated by western blotting probing cell extracts with anti-DnaK (B, upper panel). Coomassie blue staining (B, lower panel) was used as loading control. (C) Relative transcription level of dnaK gene in knockdown strain by qPCR. Values are presented as average plus standard deviation of 3 replicates. For statistical analyses, relative expression values displayed by the knockdown mutant were compared to those presented by the cells containing pMaOri.dCas9 alone (*P < 0.05).