Table 1.
Sequences and restriction sites of the primers used in this study.
| Primer | Sequence (5′ → 3′) | Restriction Site |
|---|---|---|
| pMaOri F | AGTGACACAGGAACACTTAACG | — |
| pMaOri R | TATATTCTGTCCACATTTGTGG | — |
| Cas9 F | ATCGGCGGCCGCGGTTTGCAGTCAGAGTAGAATAG | NotI |
| Cas9 R | ATCGTCTAGATTAAGAAATAATCTTCATCTA | XbaI |
| p32Cas9 F | GCATAGAGCGGCCGCGAACAAGAAAGAGTCAGAG | NotI |
| Cas9fusion | ATATCTAAGCCTATTGAGTATTTCTTATCCATAGACT CTCCTTAGTTAGG | — |
| Cas9seq1 | ATATTAAACTAATTTCGGAG | — |
| Cas9seq2 | GTAGATTCTACTGATAAAGC | — |
| Cas9seq3 | TCAGCTTCAATGATTAAACG | — |
| Cas9seq4 | TGAACGCATGACAAACTTTG | — |
| Cas9seq5 | AATTGATTAATGGTATTAGG | — |
| Cas9seq6 | ACAAAGTTTCCTTAAAGACG | — |
| Cas9seq7 | TGGGGAAACTGGAGAAATTG | — |
| Cas9seq8 | AATCAGTGAATTTTCTAAGC | — |
| Spc F | CGATTCAAACATTAAAAATCG | — |
| Spc R | CACTCACCGTATATAAATTCTC | — |
| J23LipL32 F1 | GCTCAGTCCTAGGTATAATACTAGTCTAACTAAGGAG AGTCTATG | — |
| J23LipL32 F2 | GCATAGAGAGCTCTTGACAGCTAGCTCAGTCCTAGGTA TAATACTAGTCTAAC | SacI |
| flaBLip32 F1 | TTTTTTAATTTTTGTGCTATTCTTTTTAACCTAACTAAGG AGAGTCTATG | — |
| flaBLip32 F2 | TAGAGAGCTCTTCTTTTTTTTTAATTTTTGTGCTATTCTT | SacI |
| p32min F | TAGAGAGCTCGATTTACAAAAAATTCCTAAG | SacI |
| p32 F | TAGAGAGCTCGAACAAGAAAGAGTCAGAG | SacI |
| LipL32 R | GCATAGATCTAGATATTCAAATGTAGTTTTAGG | XbaI |
| pMaOri2 F | ACGCAATGTATCGATACCGAC | — |
| pMaOri2 R | ATAGGTGAAGTAGGCCCACCC | — |
| p32XmaIF | atcgaCCCGGGGAACAAGAAAGAGTCAGAG | XmaI |
| Scaffold R | tcgaCCCGGGAAAAAAAGCACCGACTCGGTGCCACTTTTTCAAG | XmaI |
| sgRNAbgal1 F | CATACCGTGATTTTCAATTTGCCAACCTACAACCGGTTTTAGAGCTAGAA | — |
| sgRNAbgal2 F | CATACCGTGATTTTCGGGAACCACCCCGCGGTTGTGTTTTAGAGCTAGAA | — |
| sgRNAdnaK1 F | CATACCGTGATTTTCACACGAGCCGCAATTTCTTGGTTTTAGAGCTAGAA | — |
| sgRNAdnaK2 F | CATACCGTGATTTTCAGCCGGTGACGAATCAAAGAGTTTTAGAGCTAGAA | — |
| sgRNAfligF | CATACCGTGATTTTCAACCGCTACCAAAAAGATGGGTTTTAGAGCTAGAA | — |
| qPCRBgal F | ATTGTGACAGCAATGGCAAA | — |
| qPCRBgal R | GTGGGGAATAATCCACATCG | — |
| qPCR16S F | GGTGCAAGCGTTGTTCGG | — |
| qPCR16S R | GATATCTACGCATTTCACCGC | — |
*Underlined are the restriction sites.