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. 2019 Feb 12;9:1864. doi: 10.1038/s41598-018-38205-y

Figure 1.

Figure 1

Evaluation of the protective effect of secretions from endometrial epithelial cells following preincubation with TFV or TAF on HIV infection of blood CD4+ T cells. (a) Dose-dependent increase in intracellular TFV-DP levels following treatment of purified polarized endometrial (EM) epithelial cells with TAF (white bars) or TFV (black bars) for 24 hr. Bars and horizontal lines represent mean and SEM respectively from triplicate cultures of cells from a representative patient. Values are expressed as fmol/million cells. (b,c) Levels of HIV infection (released p24) in CD4+ T cells after incubation with apical and basolateral CM from EM epithelial cells pre-treated with TFV (3277 µM) or TAF (10 µM) as described in Methods. (b) Representative example of secreted p24 levels from a single patient run in quadruplicate. Columns and horizontal lines represent the mean and SEM respectively. (c) Secreted p24 values are normalized to the infection of CD4+ T cells in the absence of CM (media control) which is set to 100%. Each circle represents an individual patient (n = 10) and horizontal lines represent the mean and SEM. Blood CD4+ T cells were isolated from 4 donors. *p < 0.05. **p < 0.01.