Fig. 2.

Knockdown of LINC01234 inhibits colon cancer cell proliferation via suppressing serine/glycine metabolism. a MTT assays were used to determine the viability of negative control (NC), sh-LINC01234, serine treatment or serine treatment sh-LINC01234-transfected LoVo cells and HCT116 cells. b Stable isotope tracing experiments. LoVo cells and HCT116 cells expressing sh-NC or sh-LINC01234 were cultured in complete medium (Comp) or serine/glycine-deprived medium (-SG) for 24 h. GC-MS was used to detect the relative intracellular levels of serine (left) or glycine (right). Histobars represent the mean value of the peak area ± SD (arbitrary unit) corresponding to serine and glycine peaks on the MS chromatogram. c Colony formation assays were performed to determine the proliferation of negative control (NC), sh-LINC01234, serine treatment or serine treatment sh-LINC01234-transfected LoVo cells and HCT116 cells. The experiments were performed in triplicate. The data are represented as means ± SD from three independent experiments. *P < 0.05