Fig. 4.

LINC01234 activity is partially mediated by the positive regulation of SHMT2. a MTT assays were used to determine the viability of sh-SHMT2-transfected LoVo cells and HCT116 cells. b Colony formation assays were performed to determine the proliferation of sh-NC, sh-SHMT2, serine treatment or serine treatment sh-SHMT2-transfected LoVo cells and HCT116 cells. c Stable isotope tracing experiments. LoVo cells and HCT116 cells expressing sh-NC or sh-SHMT2 were cultured in complete medium (Comp) or serine/glycine-deprived medium (-SG) for 24 h. GC-MS was used to detect the relative intracellular levels of serine (left) or glycine (right). Histobars represent the mean value of the peak area ± SD (arbitrary unit) corresponding to the serine and glycine peaks on the MS chromatogram. d MTT assays were used to determine the viability of LoVo cells and HCT116 cells transfected with NC, sh-SHMT2 or cotransfected sh-LINC01234 and SHMT2-expressing vector. e Stable isotope-tracing experiments. LoVo cells and HCT116 cells transfected with NC, sh-SHMT2 or cotransfected sh-LINC01234 and SHMT2-expressing vector were cultured in complete medium (Comp) or serine/glycine-deprived medium (-SG) for 24 h. GC-MS was used to detect the relative intracellular levels of serine (left) or glycine (right). Histobars represent the mean value of the peak area ± SD (arbitrary unit) corresponding to the serine and glycine peaks on the MS chromatogram. The experiments were performed in triplicate. The data are represented as means ± SD from three independent experiments. *P < 0.05