Fig. 6.

Effects of miR-642a-5p on colon cancer cell proliferation in vitro. a MTT assays were used to determine the viability of negative control (NC), miR-642a-5p, serine treatment or serine treatment miR-642a-5p-transfected LoVo cells and HCT116 cells. b Colony formation assays were performed to determine the proliferation of negative control (NC), miR-642a-5p, serine treatment or serine treatment miR-642a-5p-transfected LoVo cells and HCT116 cells. c Stable isotope tracing experiments. LoVo cells and HCT116 cells transfected with NC or miR-642a-5p were cultured in complete medium (Comp) or serine/glycine-deprived medium (-SG) for 24 h. GC-MS was used to detect the relative intracellular levels of serine (left) or glycine (right). Histobars represent the mean value of the peak area ± SD (arbitrary unit) corresponding to the serine and glycine peaks on the MS chromatogram. The experiments were performed in triplicate. The data are represented as means ± SD from three independent experiments. *P < 0.05