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. 2019 Feb 12;9:1868. doi: 10.1038/s41598-018-38226-7

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of EWH on hippocampus COX-2 immunoreactivity in Al-treated rats. Immunoreactivity for COX-2 (arrows) in hippocampus of controls group (A and D), Al at 8.3 mg/kg b.w. (B) and Al at 100 mg/kg b.w. (E), EWH + Al 8.3 (C) and EWH + Al 100 (F) detected by immunohistochemistry. Scale bar 50 µm and objective X20. COX-2 score for intensity of immunoreactivity (0 to 4 + scale) (objective X40) of rats treated with AlCl₃ at 8.3 (G) or 100 (H), co-treated or not with EWH. Results are expressed as mean ± SD, n = 8, *P < 0.05 compared with their corresponding controls (two-way ANOVA and Bonferroni as post-hoc test).