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. 2019 Feb 12;10(2):136. doi: 10.1038/s41419-019-1355-1

Fig. 4. HMGB1 knockdown suppresses the level of autophagy in ESCC cells.

Fig. 4

a ESCC cells stably transfected with mRFP-GFP-LC3 and treated with siHMGB1. Laser confocal fluorescence microscopy analyses (left) and puncta-based quantification (right) for autophagosomes and autolysosomes (t test). In the merged image, yellow puncta indicate autophagosomes, while red puncta indicate autolysosomes. b TEM microscopic images of autophagosomes in TE-1 and Eca-109 transfected with siHMGB1. c Average number of autophagosomes per cell in TE-1 and ECA-109 cells (t test)