Table 1.

Radiation biologic parameters of ESCC cells in different groups

Cell lines	Group	D0 (Gy)	Dq (Gy)	N	SF2 (%)
TE-1	Control	3.34 ± 0.24	1.88 ± 0.17	1.76 ± 0.41	72.01 ± 2.50
	siHMGB1	2.47 ± 0.31a,*	1.83 ± 0.11a,*	2.10 ± 0.38a, *	69.08 ± 3.70 a,*
	siHMGB1 + EBSS	3.55 ± 0.26b,*	2.58 ± 0.21b,*	2.07 ± 0.35b,*	80.11 ± 1.70b,*
	EBSS	3.64 ± 0.27	2.81 ± 0.18	2.16 ± 0.37	83.10 ± 1.24
Eca-109	Control	3.18 ± 0.19	1.47 ± 0.31	1.59 ± 0.04	70.32 ± 3.30
	siHMGB1	2.48 ± 0.32a,*	1.44 ± 0.19a,*	1.79 ± 0.22a, *	65.71 ± 4.50a,*
	siHMGB1 + EBSS	3.88 ± 0.41b,*	2.00 ± 0.23b, *	1.66 ± 0.33b, *	75.11 ± 2.90b,*
	EBSS	3.98 ± 0.26	2.38 ± 0.21	1.82 ± 0.39	78.14 ± 1.40

Note: Radiobiological parameters (D₀, D_q, N and SF2) were calculated from the single-hit multitarget model (SF = 1−(1−e^ [−kD]) ^N) using GraphPad Prism 7.0

D₀: final slope; D_q: quasi-threshold dose; N: extrapolation number; SF2: survival fraction of 2 Gy

^*P < 0.05, t test

^asiHMGB1 vs. control

^bsiHMGB1 + EBSS vs. siHMGB1