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. 2019 Feb 6;10:155. doi: 10.3389/fimmu.2019.00155

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Copyright © 2019 Limongi, Baldelli, Checconi, Marcocci, De Chiara, Fraternale, Magnani, Ciriolo and Palamara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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GSH-C4 is able to reverse the increase of intracellular GSH levels after LPS stimulation. (A) RAW 264.7 macrophages were treated with 10 mM GSH-C4 for 2 hrs. Subsequently, GSH-C4 was removed from culture medium and the cells were stimulated with 100 ng/ml LPS for 1 hrs. After 1 hrs RAW 264.7 macrophages were treated again with 10 mM GSH-C4 or with fresh medium for 24 hrs. GSH-C4 content was assayed by HPLC. Data are expressed as means ± S.D. (n = 3; **p < 0.001). (B) GSH content was assayed by HPLC. Data are expressed as means ± S.D. (n = 4; **p < 0.001). nd, not determined. All the images reported in the figures are representative of at least three experiments that gave similar results.