Fig. 5.

ZIP7 and phosphorylation of ZIP7 (pZIP7) are increased after immortalization and activation. Analysis of ZIP7 expression in LCL (A) and CD40L-activated B cells from freshly isolated PBMC (C) showed increased ZIP7 protein levels compared to measurements in only T cell depleted PBMC or freshly isolated PBMC, respectively. Apart from that, not only the amount of ZIP7 was increased, but also phosphorylation of the ZIP7 importer (B, D). (A-D) Densitometric quantification of n = 5 (A, B) and n = 3 (C, D) is presented as mean + SEM (** p < 0.01, *** p < 0.001; Student’s t-test). (E) Immunodetection of one representative Western Blot.