Secretion of IL-1β in response to live and UV-inactivated HCMV requires the AIM2 inflammasome. (A) Immunoblot assay for caspase-1, ASC, NLRP3, IFI16, AIM2, and GAPDH in THP-1 cells transduced with Cas9 and guide RNA (gRNA) targeting HUMCYC pseudogene or in indicated CRISPR-generated knockout cells. (B) IL-1β secretion in THP-1 cells with the HUMCYC pseudogene or gene encoding an indicated protein deleted was measured following mock treatment (white) or treatment with live (gray) or UV-inactivated (black) HCMV (HCMV-TB40/E; MOI of 3) for 18 h. Values are averages ± SEM from three biological replicates. (C) IL-1β secretion in differentiated THP-1 cells following mock treatment or treatment with UV-HCMV (UV) alone or in combination with caspase-1 inhibitor (YVAD), caspase-4 inhibitor (LEVD), caspase-5 inhibitor (WEHD), or caspase-8 inhibitor (IETD) for 18 h. Values are averages ± SEM from three replicates. One-way ANOVA with Bonferroni correction was performed. **, P < 0.01.