Genotyping places the clinical isolate in the C. deuterogattii VGIIa major lineage. (A) Representative MLST tree of TEF1. The scale bar represents the number of substitutions per site. (B) Allele designations for the SEC744 clinical isolate and the C. deuterogattii VGIIa, VGIIb, and VGIIc lineages. (C) FACS analysis of C. deuterogattii R265 (haploid control), RBB59 (C. gattii diploid), and the SEC744 clinical isolate. PI-A, propidium iodide area. (D) Testing for hypermutator phenotype in R265, the msh2Δ mutant, and the SEC744 clinical isolate.