FIG 3.
Deletion analysis of the Cys-rich region within TraADK1622. (A) Domain architectures of TraA from a S. cellulosum strain (MSr7282) and a M. xanthus strain (DK1622). Cys-rich repeats are numerically labeled; note that S. cellulosum is missing C2 and C3. (B) Schematic depicting markerless in-frame deletion mutants (deleted residues listed) within the Cys-rich region of TraADK1622. The ability of these mutants to complement a ΔtraA mutant was assessed by a stimulation assay. Stimulation efficacy was judged as swarming distance of emergent flares from colony edges compared with a positive control (as described in the Fig. 2D and E legends). Wild-type stimulation, >75% efficacy; weak stimulation, ∼20% efficacy; very weak stimulation, <5% efficacy. See Table S1 for strain details.