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. 2019 Feb 6;10:154. doi: 10.3389/fimmu.2019.00154

Figure 5.

Figure 5

Soluble MULT1 augments effector functions of CD8 T lymphocytes. Splenocytes from naïve Klrk1−/− (black bars) and wild type (Klrk1+/+ white bars) mice were activated with anti-CD3, anti-CD28, IL-2, and IL-15 for 5 days and then exposed or not to soluble recombinant MULT1 for an additional 2 day culture. Cells were subsequently shortly activated and analyzed by flow cytometry for the expression of CD3, CD8, NKG2D, granzyme B, and IFNγ. (A) Representative contour plots are illustrated for the detection of NKG2D or Granzyme B and IFNγ on CD3+CD8+ T cell-gated cells from Klrk1−/− and Klrk1+/+ mice. Histograms showing IFNγ on CD3+CD8+ T cell-gated cells from Klrk1−/− and Klrk1+/+ mice according to treatment: PBS (dotted line) or MULT1 (filled line); gray filled represents isotype control. (B) Percentage of CD8 T lymphocytes expressing NKG2D, Granzyme B or IFNγ. Mean ± SEM n = 3 individual mice of one representative experiment. (C) MFI intensity for CD8 T lymphocytes expressing NKG2D, Granzyme B or IFNγ. Mean ± SEM n = 3 individual mice of one representative experiment. Statistical differences within Klrk1+/+ group without vs. with MULT1 *p < 0.05.