Figure 8.

Validation of interaction between studied miRNAs and their predicted target 3′UTRs in HEK293T cell line via Dual Luciferase Reporter Assay.

WT, wild-type sequence; MUT, sequence with mutations introduced within miRNA binding site in 3′UTR; *P < .05; **P < .01; ***P < .001; ns, not significant. The graphs present the decrease of relative luciferase activity in the presence of miRNA mimic in reference to scrambled control. Below each graph, the predicted interaction sites in miRNAs and mRNAs are shown, with indication of nucleotides in mRNAs mutated in rescue experiment. (A) Interaction between LATS2 3′UTR and hsa-miR-363-3p. (B) Interaction between PTEN 3′UTR and hsa-miR-363-3p. (C) Interaction between SOS1 3′UTR and hsa-miR-20b-5p. (D) Interaction between PTEN 3′UTR and hsa-miR-20b-5p.