Table 2.

Observed break points in streptavidin fluorescence emission signals upon addition of biotin. Uncertainties are estimated using the standard deviation.

	[biotin]: [SA] at saturation
	λem (nm)
λex (nm)	335 nm	350 nm	% diff.	N
280	3.76 ± 0.18	4.00	6 ± 4	6
290	3.82 ± 0.04	4.00	4.6 ± 1.1	9
	λem (nm)
λex (nm)	327 nm	350 nm	% diff.	N
280	3.81 ± 0.05	4.00	5.1 ± 1.2	7
290	3.69 ± 0.04	4.00	8.6 ± 1.3	16