Table 1.
Genotyping and qPCR Primers
|
Genotype
| |
|---|---|
| Tgfbr3 | 5′ – 3′ |
| Forward | TGATCTTAGTGGTAACCTCGCC |
| Reverse | CTAGCATGACAGGAATGTAC |
| Recombined | TTAGGTCGGTGCTGTCCTTGTT |
| Gnrhr GRIC | |
| Forward | GGACATGTTCAGGGATCGCCAGGC |
| Reverse | GCATAACCAGTGAAACAGCATTGCTG |
| Wild-type allele | |
| Forward | GAACTACAGCTGAATCAGTC |
| Reverse | CTAACAACAAACTCTGTACA |
| qPCR | |
|---|---|
| Rpl19 | |
| Forward | CGGGAATCCAAGAAGATTGA |
| Reverse | TTCAGCTTGTGGATGTGCTC |
| Fshb | |
| Forward | GTGCGGGCTACTGCTACACT |
| Reverse | CAGGCAATCTTACGGTCTCG |
| Cga | |
| Forward | TCCCTCAAAAAGTCCAQGAGC |
| Reverse | GAAGAGAATGAAGAATATGCAG |
| Lhb | |
| Forward | AGCAGCCGGCAGTACTCGGA |
| Reverse | ACTGTGCCGGCCTGTCAACG |
| Gnrhr | |
| Forward | CACGGGTTTAGGAAAGCAAA |
| Reverse | TTCGCTACCTCCTTTGTCGT |
Abbreviation: qPCR, quantitative PCR.