Fig. 10.

N/OFQ reversibly hyperpolarizes and decreases firing in POMC neurons in HFD-fed eGFP-POMC males. Membrane current traces showing the reversible hyperpolarization and decrease in firing caused by N/OFQ in chow-fed males (a; n = 13) and the similarity of these effects in HFD-fed males (b; n = 8). The composite bar graphs in (c, d) show the N/OFQ-induced hyperpolarization and decrease in firing in both the chow- and HFD-fed males, while the composite bar graph in e shows the resting membrane potential for both. Bars are representative of the means, while lines represent 1 SEM. *P < 0.05 relative to baseline, multi-factorial ANOVA/LSD. #p < 0.05 relative to chow, multi-factorial ANOVA/LSD