Fig. 6.

N/OFQ produces a reversible NOP receptor-mediated outward current due to activation of GIRK channels in eGFP-POMC mice. a Membrane current traces show the reversible outward current caused by 1 μM N/OFQ (n = 14), and the abolition of the outward current in the presence of BAN (n = 16). Composite I/V plot (b) and bar graph (c) illustrating the marked reduction in the N/OFQ- induced increase in slope conductance caused by BAN. Symbols represent means of the membrane current (I) seen at different command voltages (V), whereas bars represent the change in slope conductance (Δg) measured via linear regression between − 60 to − 80 mV and − 100 to − 130 mV, in the presence of N/OFQ alone or with BAN. Vertical lines indicate 1 SEM. *p < 0.05, multi-factorial ANOVA/LSD