Fig. 3.

miR-325-3p inhibited HBV antigen secretion and viral replication. Huh7–1.3 and HepG2.2.15 cells were transfected with miR-325-3p mimic or miR-NC for 48 h. (A) Levels of miR-325-3p in Huh7–1.3 and HepG2.2.15 cells were determined by qRT-PCR. (B) Culture medium from each group was collected and detected for levels of HBeAg by ELISA. (C) Culture medium from each group was harvested and measured for levels of HBsAg by ELISA. (D) Levels of released of nuclear HBV cccDNA were determined by quantitative PCR in each group. All data are presented as mean ± SEM, n = 6. ^#p < 0.05, ^##p < 0.01, ^###p < 0.001 vs. miR-NC