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. 2019 Feb 12;20:9. doi: 10.1186/s12865-019-0289-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Analysis of HCPs and residual DNA in final products (a) HCP detection by ELISA. ***P < 0.001. (b) Residual host cell DNA analysis by southern blot. Lanes 1–6: 10 ng, 1 ng, 500 pg, 250 pg, 100 pg, and 20 pg of BL21 (DE3) template DNA. Lanes 7–8: SP of untagged and His-tagged Aβ1–6 chimeric proteins. Lanes 9–10: IB of untagged and His-tagged Aβ1–6 chimeric proteins