Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Feb 12;38:69. doi: 10.1186/s13046-019-1066-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

PMC Copyright notice

Fig. 2 — Cell cycle analysis of the NGP neuroblastoma cell line. Top row: non-treated NGP cell line. Bottom row: treated (20 μM PRIMA-1^MET) NGP cell line. Gates: Green: cell cycle (20 μM Hoechst) c: G1 phase (dark blue); d: S phase (purple); e: G2/M phase (brown); f: sub-G1 phase (light blue). Cells in gates C, D, E, and F are distributed according to DRAQ7 and Cas3/7 (AlexaFluor 488) intensity. −/− live cells with stable membrane; −/+ live cells with triggered Cas3/7; +/+ dead cells with triggered Cas3/7 and nonfunctional membrane; +/− dead cells without activated Cas3/7 but with nonfunctional membrane. Results demonstrate that cells treated with PRIMA-1^MET die in all phases of the cell cycle and that PRIMA-1^MET activates apoptosis with similar intensity to non-treated control cells undergoing apoptosis