Fig. 1.
Aβ1–42 decreases cell viability and induced cell apoptosis in human neuroblastoma SH-SY5Y cells. MTT assay showed a significant decrease in the viability of SH-SY5Y cells after Aβ1–42 exposure in a dose (a) and time-dependent (b) manner. *P < 0.05, **P < 0.01 compared with control. c SH-SY5Y cells were incubated with DMEM containing 10% FBS, serum-free DMEM, or DMEM containing different concentrations of Aβ1–42 (5, 10, 20, and 40 μM) for 24 h, respectively. The apoptotic cells were detected by TUNEL staining (green). DAPI was used to determine the number of gross nuclei. Scale bar = 50 μm. d Quantitative analysis of the number of TUNEL-positive cells in c. *P < 0.05, **P < 0.01, compared with serum-free group. e FACS analysis using Annexin V/PI staining showed the increased cell apoptosis in SH-SY5Y cells treated with different concentrations of Aβ1–42 for 24 h compared with serum-free group. f The quantitative analysis of the number of apoptotic cells in e. *P < 0.05, **P < 0.01, compared with serum-free group. All the quantitative data were presented as mean ± SD of at least three independent experiments. s(−) serum-free, con control