Figure 2.

EA Promotes the Survival and Differentiation of NSC-derived Neurons in Transplanted Neural Network Scaffold In Vivo

(A) Representative confocal microscopy images from the NN and NN + EA groups 8 weeks after SCI. The dashed curves outline the interface of graft and host.

(B) Bar chart showing the quantification of the number and volume of grafted GFP⁺ cells (n = 5/group; Mann-Whitney U test, ^∗p < 0.05).

(C) Western blot analysis of GFP expression in the injury/graft site in the NN + EA and NN groups at 8 weeks after SCI (n = 5/group; Student's t test, ^∗p < 0.05).

(D–F) Bar charts showing the percentage of GFP⁺ cells differentiating into (D) MAP2⁺ neurons, (E) APC⁺ oligodendrocytes, and (F) GFAP⁺ astrocytes at 8 weeks after graft (n = 5/group; Student's t test, ^∗p < 0.05).

(G) Representative confocal microscopy images for MAP2, APC, and GFAP IF (arrows).

Scale bars: 1 mm in (A); 40 μm in (G); 10 μm in the high-magnification boxed areas in (G).