Figure 7.

Combination of NN Transplantation and EA Increased NT-3 Level and Activated TRKC/AKT Signaling Pathway

(A and B) (A) Western blot analysis and (B) quantitation of NT-3, TRKC, p-TRKC, and p-AKT protein expression in the injury/graft site of spinal cord at 8 weeks after SCI (n = 5/group). ^∗p < 0.05 indicates significant difference from the GS group; ^#p < 0.05 indicates significant difference from the GS + EA group; ^&p < 0.05 indicates significant difference from the NN group by one-way ANOVA followed by LSD t.

(C) Representative images showing the GFP⁺ cells colocalized with NT-3, TRKC, p-TRKC, and p-AKT (yellow, arrows). The boxed areas are shown with their corresponding higher magnification images.

(D) Bar charts showing the percentage of GFP⁺ cells colocalized with NT-3, TRKC, p-TRKC, and p-AKT (n = 5/group).

Student's t test, ^∗p < 0.05 indicates significant difference from the NN group by Student's t test. Scale bars, 20 μm in (C).