FIGURE 1.

Effects of different lysozyme levels on sow’s gut microbiota. (A) The microbial alpha diversity (as accessed by Shannon index) based on whole OTU table. The distribution and density of samples are displayed in violin plot. Boxes represent the interquartile ranges, the inside black plots represent the median, and circles are outliers. P-values are from Wilcoxon rank sum test. (B,C) Principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS) analysis based on the OTU table. Significant P-values of Anosim and multi-response permutation procedure (MRPP) between groups emphasize the differences in microbial community structure. (D) LEfse analysis at different microbial taxonomic levels [linear discriminant analysis (LDA) score = 3.5]. (E) Heatmap tree shows genera significantly different among groups and their phylogenic relationships. The abundance profiles are expressed by z-scores, and genera were clustered based on Bray–Curtis distance in the clustering tree. (F) T-test bar plot of significantly differed species between groups. (G,H) Spearman’s correlation networks based on genera profile. Control group (G) and 1.0 kg/t treated group (H) showed alterations in microbial relationships.