Figure 4.

The function of homeobox D4 in gastric adenocarcinoma cells. (a) Protein expression level of homeobox D4 in normal gastric cells and gastric adenocarcinoma cells. (b) Western blot results verified the transfection efficiency of homeobox D4-siRNA (si) and homeobox D4-overexpression (o/e). Overexpressing homeobox D4 enhanced protein expression of c-Myc and cyclin D1, while silencing homeobox D4 exerted opposite effects. (c) Quantitative real-time polymerase chain reaction showed that homeobox D4 regulated the expression of c-Myc and cyclin D1 from the transcription level. Overexpression of homeobox D4 promoted cell proliferation (d), migration (e), and invasion (f). *means P<0.05 by One-way ANOVA test