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. 2018 Sep 26;375(2):409–424. doi: 10.1007/s00441-018-2924-9

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© The Author(s) 2018

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

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Fig. 4 — Immunofluorescent staining of bovine (a–c) and porcine (d–f) intestinal organoids to show evidence of epithelial differentiation. (a and d) Rabbit anti-chromogranin A (ChgA—enteroendocrine cells, white arrows and insets shows examples), (b and e) Rabbit anti-mucin 2 (Muc2—goblet cells, white arrows, insets), (c and f) Expression of rabbit anti-E-cadherin (E-cad—epithelial adherens junctions, insets. Panels a–f) Green, FITC; red, rhodamine (f-actin); blue, DAPI. Original magnification × 400, scale bar represents 20 μm