Fig. 5.

Bovine (a–c) and porcine (d–f) intestinal organoids infected with 1 × 10⁶Toxoplasma gondii RH parasites. Organoids were incubated with parasites for 1 h at 37 °C, embedded in Matrigel and incubated for a further 24 h at 37 °C. Parasite surface antigen 1 (SAG1; Alexa Fluor 488—green), f-actin (rhodamine—red) and nuclei (DAPI—blue) are shown. Panels a and d and zooms (panels a′ and d′) illustrate intraluminal parasites. Panels b and e and zooms (panels b′ and e′) show parasite rosettes indicating that replication is occurring (c, c′, c″, f, f′ and f″). Image sections show intracellular parasites (white arrows). Panels a, b, d and e scale bar represents 20 μm, panels c and f, 30 μm. Original magnification × 400