Table 1.
Selected epithelial-related proteins identified by label-free mass spectrometry in bovine intestinal epithelial organoids
| Uniprot ID | Gene name | Protein name | Function |
|---|---|---|---|
| F1MEG3 | LAMA1 | Laminin subunit alpha 1 | Basal lamina protein |
| F1N2X7 | AFDN | Afadin | Cell junction development |
| Q3T0L5 | EPCAM | Epithelial cell adhesion molecule | Tight junction formation |
| Q3B7N4 | CLDN7 | Claudin-7 | |
| Q765P1 | CLDN2 | Claudin-2 | |
| F1MBN2 | TJP2 | Tight junction protein 2, ZO-2 | Tight junction protein |
| F1N2D3 | TJP1 | Tight junction protein 1, ZO-1 | |
| F1N789 | VCL | Vinculin | Focal adhesion formation |
| F1MFC2 | DSG2 | Desmoglein-2 | Hemidesmosome/desmosome proteins |
| E1BKT9 | DSP | Desmoplakin | |
| E1BFQ6 | ITGA6 | Integrin subunit alpha 6 | |
| E1B864 | ITGB4 | Integrin beta | |
| E1BF59 | PLEC | Plectin | |
| F1MU12 | KRT8 | Keratin, type II cytoskeletal 8 | Intermediate filament component |
| Q29S21 | KRT7 | Keratin, type II cytoskeletal 7 | |
| A6QQQ9 | KRT20 | Keratin, type II cytoskeletal 20 | |
| P08728 | KRT19 | Keratin, type II cytoskeletal 19 | |
| P53712 | ITGB1 | Integrin beta-1 | Maintenance of polarisation |
The presence of epithelial-specific proteins in the organoids are suggestive that the tissue is differentiating into an epithelium and is maintaining its structural integrity (n = 3)