Table 2.
Selected intestinal-related proteins identified by label-free mass spectrometry in bovine intestinal epithelial organoids
| Uniprot ID | Gene name | Protein name | Function |
|---|---|---|---|
| A6H742 | PLS1 | Plastin 1 | Actin bundling in brush border |
| Q5E9Z3 | VIL1 | Villin 1 | Enterocyte marker |
| Q56JX9 | FABP2 | Fatty acid-binding protein, intestinal | Enterocyte marker |
| P31976 | EZR | Ezrin | Epithelial organisation and villus morphogenesis |
| A6H7H6 | CDH17 | Cadherin 17 | Intestinal cell-cell adhesion |
| Q3T0I2 | CTSH | Pro-cathepsin | Intestinal homeostasis |
| F1MVJ8 | OLFM4 | Olfactomedin-4 | Intestinal stem cell marker |
| E1BAQ3 | MUC13 | Mucin 13 | Mucus component |
| F1N3J3 | AGR2 | Anterior gradient 2 | |
| G5E5Q6 | TFF3 | Trefoil factor 3 | |
| F1MB08 | ENO1 | Alpha-enolase | |
| Q5E9B7 | CLIC1 | Chloride intracellular channel protein 1 | |
| E1BBS6 | QSOX2 | Sulphhydryl oxidase | Pluripotency |
Intestine-specific proteins identified using label-free mass spectrometry are suggestive that tissue is displaying an intestinal phenotype and is differentiating into terminal cell types (n = 3)