Fig. 5.

Bmal1 deficiency is associated with high ROS production, reduced ROS defense gene expression, increased RNA oxidation and decreased catalase levels in NPCs. a Representative photomicrographs and quantification of NPCs derived from Bmal1^+/+ mice (+/+) and Bmal1^−/− mice (−/−) with ROS-sensitive dye CellROX® 24 h after seeding, **P < 0.001, n = 6 mice per genotype. Scale bar = 30 µm (b) quantification of relative ROS defense gene expression in Bmal1^+/+ mice and Bmal1^−/− mice, n = 9 mice per genotype. **P < 0.01; ***P < 0.0001. c Representative photomicrographs and quantification of 8-OH(d)G immunoreaction (IR, red) in NPCs as integrated density (I.D.), **P < 0.01, n = 5 mice per genotype. Scale bar = 20 µm d quantification of oxidized RNA (RNAox) levels after immunopurification from total RNA with 8-OH(d)G antibody. **P < 0.001, n = 6 mice per genotype. e Representative immunoblots and quantification of relative catalase and SOD2. **P < 0.01, n = 6 mice per genotype