Table 3.
Summary of edition profiles of transgenic lines.
| CRISPR-PDS apple | CRISPR-TFL1.1 apple | CRISPR TFL1.1 pear | |
|---|---|---|---|
| Total lines analyzed (#) | 41 | 5 | 5 |
| Homozygous1 lines (#) | 0 | 0 | 1 |
| Heterozygous2 lines (#) | 8 | 0 | 0 |
| Biallelic3 lines (#) | 37 | 5 | 4 |
| Chimeric lines | 36 | 5 | 4 |
| Non-edited lines | 3 | 0 | 0 |
| Total clones sequenced (#) | 174 | 20 | 20 |
| Target 1 mutated alone (#) | 53 | 0 | 0 |
| Target 2 mutated alone (#) | 6 | 0 | 0 |
| Targets 1 and 2 mutated (#) | 89 | 20 | 20 |
| Non-mutated clones (#) | 26 | 0 | 0 |
1Homozygous = both alleles are mutated and present the same mutation.
2Heterozygous = only one allele is mutated. 3Biallelic = both alleles are mutated but mutations are not identical. 4Chimeric = presence of different edited cell line profiles.