Table 1.
Precursor ions and product ions of the unlabeled and labeled Alternaria mycotoxins, optimized fragmentation conditions, retention times, and quantifier/qualifier ratio.
| Analyte | Precursor ion m/z | Product ion m/z | Q1 Pre-bias [V] | CE [V] | Q3 Pre-bias [V] | Retention time [min] | Ratio quantifier/qualifier∗ |
|---|---|---|---|---|---|---|---|
| ATX I | 351.20 | 297.20 | 25 | 28 | 19 | 9.6 | 1.15 |
| 314.20 | 27 | 32 | 21 | ||||
| ALTP | 349.30 | 261.25 | 18 | 28 | 15 | 10.2 | 0.74 |
| 303.00 | 18 | 19 | 18 | ||||
| AOH | 257.30 | 213.00 | 15 | 23 | 12 | 11.4 | 1.27 |
| 215.05 | 14 | 24 | 13 | ||||
| [2H4]-AOH | 261.30 | 217.00 | 15 | 23 | 12 | 11.4 | 2.09 |
| 218.05 | 14 | 24 | 13 | ||||
| TEN | 413.30 | 141.05 | 15 | 23 | 23 | 12.5 | 1.46 |
| 271.30 | 15 | 17 | 17 | ||||
| ATX II | 349.40 | 313.25 | 16 | 25 | 12 | 13.6 | 0.45 |
| 331.30 | 20 | 15 | 20 | ||||
| STTX III | 347.20 | 329.20 | 17 | 22 | 21 | 15.4 | 1.16 |
| 301.10 | 17 | 35 | 18 | ||||
| AME | 271.30 | 256.20 | 20 | 23 | 25 | 18.2 | 3.63 |
| 228.20 | 19 | 30 | 13 | ||||
| [2H4]-AME | 275.30 | 260.20 | 20 | 23 | 25 | 18.2 | 3.47 |
| 232.20 | 19 | 30 | 13 | ||||
| TA | 196.30 | 112.05 | 22 | 26 | 20 | 8.3 | 1.01 |
| 139.00 | 14 | 22 | 11 | ||||
| [13C6,15N]-TA | 203.25 | 113.05 | 22 | 26 | 20 | 8.3 | 0.91 |
| 142.00 | 14 | 22 | 11 |
∗Mean of five injections. The quantifier/qualifier ratios were determined in pure solvents.