Fig. 7.
GPR35 deficiency affects the expression of cell junction molecules. Colorectal epithelial cells (Caco2) were transfected with siRNA targeting GPR35 (GPR35 KD) or non-targeting siRNA which was used as control (control). Then the cells were treated with or without 1% DSS for 4 h (n = 3 biological repeats). The expression of ZO-1, E-cadherin, and Claudin1 was decreased in GPR35 deficiency cells. DSS treatment resulted in decrease in ZO-1, E-cadherin, and Claudin1, but the decreases in ZO-1 and Claudin1 were more severe in GPR35 knockdown cells