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. 2019 Feb 6;10:188. doi: 10.3389/fmicb.2019.00188

FIGURE 2.

FIGURE 2

RT-qPCR analysis of relative expression of selected genes related to carbon metabolism, sulfate reduction, electron transfer and biofilm formation in D. vulgaris biofilms under saline and freshwater conditions. (A) Relative expression of carbon metabolism enzymes lactate dehydrogenase ldh, pyruvate formate lyase DVU2272 and pyruvate dehydrogenase DVU3025 in the primary left y-axis, and formate dehydrogenase DUV0588 in the secondary right y-axis. (B) Relative expression of dissimilatory sulfite reductase dsrB, dsrC, adenosine 5′-phosphosulfate reductase aprA, aprB and pyrophosphatase ppaC in the primary left y-axis, and dissimilatory sulfite reductase alpha subunit dsrA and sulfate adenylytransferase Sat in the secondary right y-axis. (C) Relative expression of Fe hydrogenase hydA, NiFeSe hydrogenase hysA-1, Ech hydrogenases echE, formate dehydrogenase DVU1817and c3-type cytochromes DVU3171 and DVU2524 in the primary left y-axis, as well as NiFe hydrogenase hynA-1, Ech hydrogenase echF, and c3-type cytochrome DVU2809 in the secondary right y-axis. (D) Relative expression of exopolysaccharide synthesis protein DVU0281 in the primary left y-axis and sensor histidine kinase response regulator DVU3062 in the secondary right y-axis. Relative expression refers to the transcript level of a specific gene normalized with that of reference gene recA. Results are presented as mean ± standard deviation (n = 3). Significant difference: ∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001.