(A) Venn diagram of genes differentially expressed and spliced in
Mx1-Cre+
Srsf2P95H/+
Sf3b1K700E+ cells relative to
Mx1-Cre+ WT cells in any
replicate.
(B) Expression of Mpl, Itga2b, and Pbx1 in lineage-
c-Kit+ (LK) cells from
Mx1-Cre+
Srsf2P95H/+
Sf3b1K700E/+ mice relative to control groups (n =
8–10 mice per genotype). Error bars represent mean ± SD. ***p
< 0.001, ****p < 0.0001 versus
Mx1-Cre+ WT; ####p < 0.0001
versus Mx1-Cre+
Srsf2P95H/+; AAp < 0.01,
AAAp < 0.001, AAAAp < 0.0001 versus
Mx1-Cre+
Sf3b1K700E/+.
(C) GO enrichment analysis of Mx1-Cre+
Srsf2P95H/+, Mx1-Cre+
Sf3b1K700E/+, and
Mx1-Cre+
Srsf2P95H/+
Sf3b1K700E/+ cells relative to
Mx1-Cre+ WT cells. Circle size indicates the
magnitude of the p value for each term and comparison.
(D) Immunofluorescence of nuclear phosphorylated-p65 (p-p65) level in
LK cells from Vav-Cre+ WT,
Vav-Cre+
Srsf2P95H/+, Vav-Cre+
Sf3b1K700E/+, and
Vav-Cre+
Srsf2P95H/+
Sf3b1K700E/+ mice following LPS stimulation
ex vivo. Scale bars, 10 mm.
(E) Violin plots quantifying nuclear p-p65 intensity of LK cells from
(D). ANOVA and Kruskal-Wallis ranked test was performed and adjusted for false
discovery rate.
(F) Schema of competitive BMT using BM MNCs from
Vav-Cre+ WT,
Vav-Cre+
Srsf2P95H/+, and
Vav-Cre+
Sf3b1K700E/+ mice after chronic LPS exposure.
(G) Percentage of CD45.2 chimerism in peripheral blood of recipients (n
= 4–5 mice per group) from primary and secondary BMT (both at 10 weeks
post BMT).
(H) Chimerism of LSK or myeloid progenitor (MP; lineage-
Sca-1- c-Kit+) fractions from primary recipients 14
weeks post BMT.
(I) Kaplan-Meier analysis of Mx1-Cre+ WT,
Mx1-Cre+
Srsf2P95H/+, and
Mx1-Cre+
Sf3b1K700E/+ mice after a single dose of LPS (15
mg/kg) in vivo. Log-rank Mantel-Cox test was performed. p =
0.0055.
ANOVAand Tukey’s post hoc test were used to compare groups. *p
< 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001
versus Uav-Cre+WT mice(“#” denotes versus
Vav-Cre+
Srsf2P95H/+ mice and
“Λ” denotes versus
Vav-Cre+
Sf3b1K700E/+ mice). In (G) and (H), the top and
bottom lines of the box represent the upper and lower quartiles, respectively;
the line inside the box represents the median; the lines above and below the box
represent the maximum and minimum values, respectively. See also Figure S5 and Table S6.