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. Author manuscript; available in PMC: 2019 Aug 1.
Published in final edited form as: J Mol Cell Cardiol. 2018 Jun 7;121:51–59. doi: 10.1016/j.yjmcc.2018.06.002

Figure 1. cpTOME and Forskolin stimulate PI4P hydrolysis in NRVMS, but Isoproterenol does not.

Figure 1

NRVMs were transduced with GFP-FAPP-PH adenovirus overnight before imaging in 1% FBS containing media. A) Representative images of Fsk (10μM)- and cpTOME (10μM) - induced PI4P hydrolysis. Bar-10μm B) Average data cpTOME (10μM), C) Average data Iso (1 μM), D) Average data Fsk (10μM), induced PI4P hydrolysis. Images taken from n=6 cells each from 6 separate preparations of NRVMs. All time course graphs are presented as mean ± standard error. Agonist treatments were compared to vehicle control performed on the same day and were added where indicated by the arrow. All data was analyzed by two way unpaired ANOVA with Sidak’s post-hoc test. ** p<0.001 *** p<0.0001 **** p<0.00001