Figure 6.

DAPT Treatment Increased the Efficiency of NGN2-Induced Neuronal Reprogramming from Postnatal Astrocytes

(A) The knockdown efficiency of DATP in postnatal astrocytes was determined by western blotting. Quantitative analysis showed no significant difference in the knockdown efficiency of DAPT between cortex (CTX), cerebellum (CE), and spinal cord (SC) astrocytes. n = 3 for each group. ^∗∗∗p < 0.001 (Student's t test); p ≥ 0.05, no statistically significant difference (n.s.) (ANOVA Tukey's post hoc test).

(B) Experimental scheme of blocking NOTCH1 signaling in astrocytes by DAPT during neuronal reprogramming. AM, astrocyte culture medium; NM, neuronal induction medium.

(C) Representative pictures of NGN2-induced neurons with or without DAPT treatment by staining with Tuj1 at 12 dpi.

(D) The efficiency of NGN2-induced neuronal reprogramming in the DAPT group compared with control group (n = 3; cells = 1,000–1,700 for each condition). ^∗p < 0.05 by ANOVA Tukey's post hoc test. Scale bar, 100 μm.