Figure 7.

Interference of NOTCH1 Signaling Increased the Efficiency of NGN2-Induced Neuronal Reprogramming from Postnatal Astrocytes

(A) Western blotting analysis of the knockdown efficiency of Notch1 shRNA in postnatal cortex astrocytes. n = 3 for each group. ^∗∗p < 0.01, ^∗∗∗p < 0.001 by ANOVA Tukey's post hoc test.

(B) The knockdown efficiency of Notch1 shRNA in postnatal astrocytes was determined by western blotting. Quantitative analysis showed no significant difference in the knockdown efficiency of Notch1 shRNA between cortex (CTX), cerebellum (CE), and spinal cord (SC) astrocytes. n = 3 for each group. ^∗∗∗p < 0.001 (Student's t test); p ≥ 0.05, no statistically significant difference (n.s.) (ANOVA Tukey's post hoc test).

(C) Experimental scheme of Notch1 knockdown in astrocytes by lentivirus-mediated shRNA during neuronal reprogramming.

(D) Representative pictures of NGN2-induced neurons with or without shRNA interference by staining with Tuj1 at 12 dpi.

(E) The efficiency of NGN2-induced neuronal reprogramming in Notch1 shRNA group compared with control group (n = 3; cells = 1,000–1,700 for each condition). ^∗p < 0.05 by ANOVA Tukey's post hoc test. Scale bar, 100 μm.