Table 3.
List of the antibodies and the antisera that were used in the experiments
| Antibody | Antigen | Source | Species | Dilution |
|---|---|---|---|---|
| PV235 | Purified carp parvalbumin | Swant Inc., Marly, Switzerland | Mouse monoclonal Lot 10-11F |
1:1000–5000 |
| PV25 | Recombinant rat parvalbumin | Swant Inc., Marly, Switzerland | Rabbit polyclonal Lot 5.10 |
1:1000–1:5000 |
| GP72 | Recombinant mouse parvalbumin | Swant Inc., Marly, Switzerland | Guinea pig polyclonal | 1:1000–1:5000 |
| PVG213/214 | Recombinant rat parvalbumin | Swant Inc., Marly, Switzerland | Goat polyclonal | 1:1000 |
| GFP | Recombinant peptide | Molecular Probes, Waltham, (USA) | Rabbit polyclonal | 1:3000 |
| 5-HT | Serotonin | Immunonuclear | Rabbit polyclonal | 1:2000 |
| TH | Tyrosine-hydroxylase | Immunostar, Stillwater (USA) | Rabbit polyclonal | 1:10,000 |
| SMI-32 | Non-phosphorylated filaments | Millipore, USA | Mouse monoclonal | 1:1000 |
| VGlut 1 | Recombinant peptide | Synaptic System, Germany | Rabbit or mouse | 1:5000/1:20,000 |
| VGlut 2 | Recombinant peptide | Synaptic System, Germany | Rabbit | 1:10,000 |
| GAD | Recombinant peptide | Millipore, USA | Mouse | 1:2000 |
The antibodies against Parv were utilized primarily to confirm that the endings from the OFC did indeed impinge on parvalbumin-immunoreactive neurons in the parvafox nucleus. The serotonin- and TH-antisera helped to define the borders of the raphe and the coeruleus nuclei. GFP-antisera served to enhance the fluorescence in the terminals of the brainstem and the spinal cord