Figure 6.

Ly6G ligation impairs neutrophil attachment to endothelium. (A-B) Mice were treated with 5 µg of 2A3 or 1A8. Four hours later, leukocyte arrest in postcapillary venules of the cremaster muscle is induced by intra-arterial injection of 1 µg of CXCL1. (A) Duration of attachment of neutrophils on the endothelium in seconds. (B) Rolling flux was quantified as the number of leukocytes rolling past an arbitrary landmark, counted in 1-minute segments and then normalized to the leukocyte rolling flux prior to CXCL1 injection; n = 5 mice per group. ***P ≤ .001.